Journal: Journal of Clinical Medicine

Article Title: Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing

doi: 10.3390/jcm9113458

Figure Lengend Snippet: List of DEGs when the IgG4-ROD tissue was compared to all control tissues.

Article Snippet: Immunostaining by mouse anti-human MMP12 (1:50, MAB919, R&D Systems, Minneapolis, MN, USA) and rabbit anti-human SPP1 (1:1000, HPA027541, Atlas Antibodies, Bromma, Sweden) was performed using paraffin-embedded biopsy tissues.

Techniques: Control

Journal: Journal of Clinical Medicine

Article Title: Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing

doi: 10.3390/jcm9113458

Figure Lengend Snippet: Differentially expressed genes of IgG4-ROD versus orbital MALT lymphoma: top 40 in descending p value.

Article Snippet: Immunostaining by mouse anti-human MMP12 (1:50, MAB919, R&D Systems, Minneapolis, MN, USA) and rabbit anti-human SPP1 (1:1000, HPA027541, Atlas Antibodies, Bromma, Sweden) was performed using paraffin-embedded biopsy tissues.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing

doi: 10.3390/jcm9113458

Figure Lengend Snippet: Differentially expressed genes of IgG4-ROD versus RLH: top 40 in descending p value.

Article Snippet: Immunostaining by mouse anti-human MMP12 (1:50, MAB919, R&D Systems, Minneapolis, MN, USA) and rabbit anti-human SPP1 (1:1000, HPA027541, Atlas Antibodies, Bromma, Sweden) was performed using paraffin-embedded biopsy tissues.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing

doi: 10.3390/jcm9113458

Figure Lengend Snippet: Results of real-time PCR for validation of differentially expressed genes obtained from RNA sequencing. *** Messenger RNA of MMP12 and SPP1 in immunoglobulin G4-related ophthalmic disease is significantly higher than mucosa-associated lymphoid tissue lymphoma ( p = 2.7141 × 10 −8 and 1.2044 × 10 −7 , respectively). Statistical analysis was performed by a Mann–Whitney U test ( n = 30). IgG4-ROD: Immunoglobulin G4 related ophthalmic disease, MALT: Mucosa-associated lymph tissue, MMP12: Matrix metallopeptidase 12, SPP1: Secreted phosphoprotein 1.

Article Snippet: Immunostaining by mouse anti-human MMP12 (1:50, MAB919, R&D Systems, Minneapolis, MN, USA) and rabbit anti-human SPP1 (1:1000, HPA027541, Atlas Antibodies, Bromma, Sweden) was performed using paraffin-embedded biopsy tissues.

Techniques: Real-time Polymerase Chain Reaction, Biomarker Discovery, RNA Sequencing, MANN-WHITNEY

Journal: Journal of Clinical Medicine

Article Title: Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing

doi: 10.3390/jcm9113458

Figure Lengend Snippet: Representative immunohistochemical staining patterns of formalin-fixed, paraffin-embedded sections (MMP12: magnification 400×, bar: 50 μm, SPP1: magnification 200×, bar: 100μm). The upper row is immunoreactivity of MMP12, and the lower row is immunoreactivity of SPP1. From left column to right column, IgG4-ROD, MALT lymphoma, RLH, and the lacrimal gland tissue section are shown. Many MMP12 positive cells are observable in the fibrotic areas and follicular areas of IgG4-ROD tissues. SPP1 is intensely stained in the fibrotic part of IgG4-ROD. IgG4-ROD: Immunoglobulin G4 related ophthalmic disease, MALT: Mucosa-associated lymph tissue, RLH: Reactive lymphoid hyperplasia, MMP12: Matrix metallopeptidase 12, SPP1: Secreted phosphoprotein 1.

Article Snippet: Immunostaining by mouse anti-human MMP12 (1:50, MAB919, R&D Systems, Minneapolis, MN, USA) and rabbit anti-human SPP1 (1:1000, HPA027541, Atlas Antibodies, Bromma, Sweden) was performed using paraffin-embedded biopsy tissues.

Techniques: Immunohistochemical staining, Staining, Formalin-fixed Paraffin-Embedded